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Evaluation of the use of microemulsions as eluents in high‐performance liquid chromatography
Author(s) -
ElSherbiny Dina Tawfik Mohamed,
ElAshry Saadia Mohamed,
Mustafa Mohamed Ahmed,
AbdElRahman ElEmam Ali,
Hansen Steen Honoré
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390067
Subject(s) - chromatography , high performance liquid chromatography , microemulsion , chemistry , reversed phase chromatography , dilution , pulmonary surfactant , biochemistry , physics , thermodynamics
An overview of the use of microemulsions as eluents in HPLC is given. The separation mechanisms involved are discussed and the technique is compared to solvent modified micellar chromatography as well as to ordinary reversed‐phase HPLC in order to evaluate differences and similarities. The microemulsion systems have been used for the separation of a number of antihypertensives and non steroidal antiinflammatory drug substances as well as for the analysis of bumetanide and acebutolol in plasma and urine. The plasma samples may be injected directly onto the HPLC system after a simple dilution.