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Feed additives in animal nutrition: Quantification of a new adrenergic drug by hyphenated techniques
Author(s) -
Bocca Beatrice,
Cartoni Claudia,
Mattia Michela Di,
Fiori Maurizio,
Felli Marialinda,
Neri Bruno,
Russo Mario Vincenzo,
Brambilla Gianfranco
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390045
Subject(s) - chemistry , chromatography , mass spectrometry , extraction (chemistry) , gas chromatography , electron capture detector
Zilpaterol, (±)‐ trans ‐4,5,6,7‐tetrahydro‐7‐hydroxy‐6‐(isopropylamino) imidazo [4,5,1‐ jk ]‐[1]benzazepin‐2(1 H )‐one, is an adrenergic agonist drug licensed in Mexico and South Africa as feed additive for cattle at slaughter age. In contrast, the EU legislative framework prohibits the use of beta‐agonists as growth promoters to produce lean meat. In this study, the compound was quantified as its TMS derivative both by gas chromatography‐mass spectrometry (GC‐MS) with a quadrupole detector (QUADR) and by GC‐MS/MS with an ion trap detector (ITD), in Electron Impact (EI) ionization. Subsequently, an extraction and clean‐up procedure for the identification of Zilpaterol in commercial feeds was set up. Different extraction solvents and matrices were considered for their influence on Zilpaterol yield. The analytical method led to good recoveries and limits of detection and quantification in feeds well below the 5–20 μg/g dose proposed for animal nutrition.