Premium
Marfey's reagent: Past, present, and future uses of 1‐fluoro‐2,4‐dinitrophenyl‐5‐L‐alanine amide
Author(s) -
B'Hymer Clayton,
MontesBayon Maria,
Caruso Joseph A.
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390019
Subject(s) - reagent , derivatization , chemistry , enantiomer , amide , alanine , dinitrophenyl , amino acid , amine gas treating , chromatography , chiral derivatizing agent , combinatorial chemistry , enantiomeric excess , organic chemistry , enantioselective synthesis , high performance liquid chromatography , catalysis , chiral column chromatography , biology , biochemistry , antibody , immunology
This article describes some of the uses of Marfey's reagent, 1‐fluoro‐2,4‐dinitrophenyl‐5‐L‐alanine amide (FDAA), a pre‐column derivatizing reagent for the separation of enantiomeric isomers of amino acids and amine compounds. An introduction to the basic implementation of this reagent and its historical development are presented. Actual uses in amino acid, short peptide, and pharmaceutical compounds are included, as well as the advantages and disadvantages over other pre‐column derivatization techniques and direct chromatographic separations. Applications of current interest, including its use as an orthogonal analysis and in enantiomeric purity analysis of selenoamino acids using element specific detection, are also discussed.