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Separation of basic central nervous system drugs by capillary electrochromatography
Author(s) -
Jiskra Jan,
Claessens Henk A.,
Cramers Carel A.
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200390013
Subject(s) - capillary electrochromatography , derivatization , electrochromatography , ethylenediamine , chemistry , chromatography , acetonitrile , capillary electrophoresis , high performance liquid chromatography , organic chemistry
This article describes the sample preparation and separation of three strongly basic central nervous system (CNS) drugs by capillary electrochromatography. The separation was developed on Hypersil C8 MOS and Hypersil Phenyl stationary phases using acetonitrile (ACN) as organic modifier together with mobile phase additives, ammonia, ethylenediamine, or 1,3‐diaminopropane. A successful, fast separation after a simple derivatization procedure of the compound was achieved on the Hypersil Phenyl stationary phase using ethylenediamine as mobile phase additive. The general approach to method development for complex mixtures is discussed.