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Separation of racemic 2,4‐dinitrophenyl amino acids on zirconia‐immobilized quinine carbamate in reversed‐phase liquid chromatography
Author(s) -
Park Jung Hag,
Lee Joon Woo,
Song Young Tae,
Ra Chun Sup,
Cha Jin Soon,
Ryoo Jae Jeong,
Lee Wonjae,
Kim In Whan,
Jang Myung Duk
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301713
Subject(s) - chemistry , carbamate , enantiomer , selectivity , chromatography , amino acid , organic chemistry , cubic zirconia , high performance liquid chromatography , lewis acids and bases , silica gel , catalysis , ceramic , biochemistry
Abstract Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate‐coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP‐amino acids in reversed‐phase liquid chromatography. Retention and enantioselectivity of this phase were compared to those for quinine carbamate bonded onto silica. Most amino acids studied were separated on the quinine carbamate‐zirconia CSP although retention was longer and chiral selectivity was somewhat lower than on the corresponding silica CSP. Increased retention and decreased selectivity are probably due to strong non‐enantioselective Lewis acid‐base interactions between the amino acid molecule and the residual Lewis acid sites on the zirconia surface.