Premium
Reduced‐bore monolithic silica column modified with C 8 ‐TEOS for reversed‐phase electrochromatography
Author(s) -
Qu Qishu,
Qu Rongjun,
Xu Qiang,
Wang Yubao,
Wang Xiaorong,
He Youzhao
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301699
Subject(s) - capillary electrochromatography , electrochromatography , monolithic hplc column , chromatography , acetonitrile , sol gel , materials science , silica gel , analytical chemistry (journal) , chemistry , high performance liquid chromatography , capillary electrophoresis , nanotechnology
Monolithic silica columns of 2.7 mm ID were prepared and derivatized with C 8 ‐TEOS and TEOS by on‐column sol‐gel reaction. These C 8 large diameter monolithic silica columns gave 21 000 theoretical plates for aromatic hydrocarbons in 60% acetonitrile and 40% Tris‐HCl buffer. The surface areas as well as the separation reproducibility were improved on coating by the sol‐gel approach. Joule heating was greatly reduced by using monolithic columns to which fine quartz sand had been added during column preparation. Since this is a preliminary investigation on a monolithic column with such a large inner diameter, the separation efficiency was not so high as that presently achieved in normal capillary electrochromatography (CEC). However, use of the columns improved sample loadability and concentration detectability of electrochromatography, and semi‐preparative separations could be performed.