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Sample preparation followed by HPLC under harmless 100% aqueous conditions for determination of oxytetracycline in milk and eggs
Author(s) -
Furusawa Naoto
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301688
Subject(s) - high performance liquid chromatography , chromatography , oxytetracycline , chemistry , sample preparation , aqueous solution , antibiotics , organic chemistry , biochemistry
A simple and hazardous chemical‐free method for the high‐performance liquid chromatographic determination of oxytetracycline (OTC) residues in milk and eggs has been developed. Sample preparation consists in homogenization with an aqueous solution by means of a handheld ultrasonic homogenizer followed by centrifugal ultrafiltration. HPLC is performed with an isocratic aqueous mobile phase and a photo‐diode array detector. Average recoveries of OTC (0.05, 0.1, and 0.2 μg mL –1 for milk; 0.1, 0.2, and 0.4 μg mL –1 for eggs) were ≥ 84% with relative standard deviations of ≤ 2.3%. The total time required for the analysis of one sample and LOQs were < 30 min and < 0.1 μg mL –1 , respectively. In all the processes, no organic solvents or hazardous reagents were used.