Premium
Analysis of basic compounds at high pH values by reversed‐phase liquid chromatography
Author(s) -
Stella Cinzia,
Rudaz Serge,
Mottaz Manuel,
Carrupt PierreAlain,
Veuthey JeanLuc
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301671
Subject(s) - silanol , chemistry , analyte , chromatography , phase (matter) , reversed phase chromatography , ion exchange , denticity , resolution (logic) , high performance liquid chromatography , analytical chemistry (journal) , ion , organic chemistry , catalysis , artificial intelligence , computer science , crystal structure
Reversed phase high performance liquid chromatography (RPLC) is currently the method of choice for the analysis of basic compounds. However, with traditional silica materials, secondary interactions between the analyte and residual silanols produce peak tailing which can negatively affect resolution, sensitivity, and reproducibility. In order to reduce these secondary interactions, which comprise ion exchange, hydrogen bonding, and London forces interactions, chromatographic analyses can be carried out at low or high pH values where silanol groups and basic compounds are mostly uncharged. The chromatographic behaviour of a particular bidentate stationary phase, Zorbax Extend C 18 , was studied with a set of basic and neutral compounds. Thanks to a higher chemical stability than traditional silica based supports, analyses were carried out with a high pH mobile phase, which represents a good alternative to the acidic mobile phases generally used to reduce ion exchange interactions. The performance of this bidentate stationary phase was also compared with that of other supports and it was proved that it is advantageous to work with high pH mobile phases when analyzing basic compounds.