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Rapid and high‐resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high‐performance liquid chromatography
Author(s) -
Bamba Takeshi,
Fukusaki Eiichiro,
Nakazawa Yoshihisa,
Kobayashi Akio
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301664
Subject(s) - chromatography , homologous series , high performance liquid chromatography , high resolution , monolithic hplc column , resolution (logic) , chemistry , column (typography) , silica gel , materials science , organic chemistry , computer science , telecommunications , remote sensing , frame (networking) , artificial intelligence , geology
A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS‐bonded silica column in the high‐performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high‐resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.