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Characterization of cigarette smoke condensates by comprehensive two‐dimensional gas chromatography/time‐of‐flight mass spectrometry (GC×GC/TOFMS) Part 2: Basic fraction
Author(s) -
Lu Xin,
Zhao Mingyue,
Kong Hongwei,
Cai Junlan,
Wu Jianfang,
Wu Ming,
Hua Ruixiang,
Liu Jianfu,
Xu Guowang
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301659
Subject(s) - chemistry , time of flight mass spectrometry , mass spectrometry , quinoline , chromatography , two dimensional chromatography , fraction (chemistry) , gas chromatography , sidestream smoke , structural isomer , cigarette smoke , gas chromatography–mass spectrometry , alkyl , pyridine , organic chemistry , smoke , ionization , toxicology , ion , biology
Abstract Cigarette smoke condensate is a complex chemical matrix. Analysis of nitrogen‐containing compounds present therein is very difficult because of the limitation of the peak capacity of conventional one‐dimensional chromatography. Extensive and laborious sample preparation is frequently required or selective detectors are frequently used. In this study, the basic fraction of mainstream cigarette smoke condensate has been investigated by using comprehensive two‐dimensional gas chromatography/time‐of‐flight mass spectrometry (GC×GC/TOFMS). Auto data processing by TOFMS software combined with manual identification was used to assign the components. 377 nitrogen‐containing compounds, including 155 pyridine derivatives, 104 quinoline/isoquinoine derivatives, and 56 pyrazine derivatives were tentatively identified. By selecting appropriate unique masses and in the light of the component positions in the structured chromatogram, alkyl‐substituted pyridines, pyrazines, and quinolines/isoquinolines were separately shown and further validated. The peaks of eight individual positional isomers of two‐carbon‐substituted pyridines and thirteen positional isomers of methyl‐substituted quinolines/isoquinolines were further confirmed, based on linear incremental retention behavior in combination with TOFMS and the structured chromatogram of GC×GC.