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Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets
Author(s) -
Šatínský Dalibor,
Neto Isabel,
Solich Petr,
Sklenářová Hana,
Conceição M.,
Montenegro B. S. M.,
Araújo Alberto N.
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301644
Subject(s) - repeatability , chromatography , chemistry , high performance liquid chromatography , caffeine , acetonitrile , detection limit , injection port , reproducibility , benzoic acid , dosage form , phosphate buffered saline , materials science , endocrinology , medicine , organic chemistry , composite material
In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed‐phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith® Flash RP‐18e, 25‐4.6 mm column (Merck, Germany) and a FIAlab® 3000 system (USA) with an 8‐port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile‐(0.01 M) phosphate buffer (10 : 90, v/v ) pH 4.05, flow rate 0.6 mL min –1 . UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity ( r >0.999) for all compounds, detection limits in the range 0.3–0.8 μg mL –1 , repeatability (RSD) of peak heights between runs in the range 1.10–4.30% at three concentration levels and intra‐day repeatability of the retention times in the range 0.28–0.43%. The analysis time was < 6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.