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Impact of biological factors on binding media identification in art objects: Identification of animal glue in the presence of Aspergillus niger
Author(s) -
Tsakalof Andreas K.,
Bairachtari Kyriaki A.,
Aslani Ioanna S.,
Chryssoulakis Ioannis D.,
Kolisis Fragiskos N.
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301627
Subject(s) - aspergillus niger , petri dish , glue , microorganism , fungus , food science , identification (biology) , chemistry , incubation , aspergillus , biology , chromatography , microbiology and biotechnology , biochemistry , materials science , botany , bacteria , composite material , genetics
Abstract The materials and especially organic materials used for creation of art objects can be utilized by various microorganisms for their growth and facilitate the microbial colonization of the object. An understanding of the chemical alterations in artefacts caused by the presence of microorganisms can be crucial for correct identification of the materials initially used for the artefact creation – nowadays an important step in restoration and/or art‐historical investigation of the art object. The present article describes a model experiment in which we investigated the possible chemical alterations in animal glue films used as substrate for growth of the fungus Aspergillus niger . The sterilized animal glue solution was poured into Petri dishes, inoculated with Aspergillus niger , and subsequently incubated at 15°C for 0, 7, 9, 14, and 28 days. After interruption of incubation, the content of the Petri dish was analyzed for amino acid composition by the GC‐MS based method. It was found that the growth of Aspergillus niger on animal glue films did not cause significant changes in the amino acid composition of the film and had no impact on animal glue identification.