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Parallel separations of oligonucleotides with optically gated sample introduction on multi‐channel microchips
Author(s) -
Xu Hongwei,
Roddy Elizabeth S.,
Roddy Thomas P.,
Lapos Julie A.,
Ewing Andrew G.
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301593
Subject(s) - oligonucleotide , multiplexing , resolution (logic) , chromatography , channel (broadcasting) , sample preparation , chemistry , pharmacogenomics , dna , analytical chemistry (journal) , biology , computer science , genetics , artificial intelligence , telecommunications , biochemistry , computer network
Abstract With the release of the human genome sequence, there has been increasing attention given to other genetic analyses, including the detection of genetic variations and fast sequencing of multiple samples for pharmacogenomics studies. Rapid injections of samples in multiplexed separation channels by optically gated sample introduction are shown here for DNA separation. Serial separations of four amino acids are shown in less than four seconds on a microchip with four multiplexed channels. Five short oligonucleotides have also been rapidly separated in 2% LPA with four channels using this technique. In addition, multiple unique samples have been simultaneously separated and five‐base resolution has been demonstrated.