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Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction
Author(s) -
Zhang Kai,
Gao Ruyu,
Jiang Zhengjin,
Yao Chuanyi,
Zhang Zhichao,
Wang Qinsun,
Yan Chao
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301590
Subject(s) - capillary electrochromatography , electrochromatography , chemistry , capillary electrophoresis , chromatography , electrophoresis , selectivity , peptide , solvent , analytical chemistry (journal) , organic chemistry , catalysis , biochemistry
A pressurized capillary electrochromatography (pCEC) instrument with solvent gradient capability has been used for the separation of a peptide mixture. Retention mechanism and selectivity of the peptides were studied by pCEC using a strong cation exchange (SCX) column. The effects of applied voltage, supplementary pressure, organic modifier concentration, ionic strength, and pH value on pCEC separation were investigated. It was found that the retention mechanism of the peptides in this system is based on a mixed mode of hydrophilic interaction, strong cation exchange, and electrophoresis. Compared with the separation results obtained by reverse phase pCEC and capillary electrophoresis (CE), this mixed‐mode pCEC is more powerful for the separation of hydrophilic peptides with similar charge‐to‐mass ratio.