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Simultaneous determination of digoxin and digitoxin by micellar electrokinetic chromatography and application to drug formulations
Author(s) -
Tseng HsiuHui,
Wu HsinLung,
Lin ShunJin,
Chen SuHwei
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301573
Subject(s) - digitoxin , chemistry , digoxin , chromatography , micellar electrokinetic chromatography , sodium dodecyl sulfate , detection limit , pulmonary surfactant , electrokinetic phenomena , tris , sodium , isopropyl alcohol , buffer solution , organic chemistry , heart failure , medicine , biochemistry
A simple micellar electrokinetic chromatographic method is described for simultaneous determination of digoxin and digitoxin. The simultaneous analysis of digoxin and digitoxin was performed in Tris buffer (10 mM; pH 9) with 90 mM sodium dodecyl sulfate and 10% isopropyl alcohol as an anionic surfactant and organic modifier. Under these conditions, good separation with high efficiency is achieved in short analysis times. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. The linear range of the method for the determination of digoxin and digitoxin was over 0.01–0.3 mg/mL; the detection limit (signal to noise ratio = 3; injection 3.5 kPa 3 s) was 4 and 6 μg/mL, respectively. Application of the proposed method to the determination of digoxin in commercial tablets and in injections proved to be feasible.