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Development of a solid phase extraction method for simultaneous determination of corticoids and tranquilizers in serum samples
Author(s) -
Quintana M. Carmen,
Ramos Lourdes,
González M. Jose,
Blanco Manuel H.,
Hernández Lucas
Publication year - 2004
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301569
Subject(s) - chromatography , repeatability , chemistry , solid phase extraction , betamethasone , analyte , extraction (chemistry) , phenylbutazone , elution , pharmacology , medicine
A simple and fast solid phase extraction (SPE) method allowing the preconcentration, clean‐up, and subsequent separate elution of phenothiazines (chlorpromazine hydrochloride, acetopromazine, and propionylpromazine hydrochloride) and glucocorticoids (dexamethasone, betamethasone acetate, and phenylbutazone) from serum samples has been developed. Both fractions were separately collected and analyzed without any additional treatment by high performance liquid chromatography with UV‐Vis. The performance of the complete procedure was satisfactory irrespective of the spiking level with recoveries in the range 64–85% for all analytes investigated but for phenylbutazone (20%). Repeatability, evaluated as the relative standard deviation, was globally better than 12%. LC‐MS was used for final confirmation of the results.