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Open tubular liquid chromatographic separations using an aptamer stationary phase
Author(s) -
Clark Stacey L.,
Remcho Vincent T.
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301512
Subject(s) - aptamer , chemistry , chromatography , oligonucleotide , molecule , phase (matter) , small molecule , high performance liquid chromatography , stationary phase , dna , organic chemistry , biochemistry , genetics , biology
Aptamers are oligonucleotides which are isolated in vitro based on their affinity for a target molecule. These molecules hold promise for use in analytical methods which rely on molecular recognition. In this study, we report the immobilization of an RNA aptamer on the inner walls of fused silica capillaries. Due to the small size of these molecules, the resulting surface coverage of these capillaries made them suitable for use in open tubular liquid chromatographic studies. These capillaries also exhibited stability when an organic modifier was added to the mobile phase. These characteristics of aptamers make them desirable over traditional affinity chromatography stationary phases.