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Determination of prednisolone, naphazoline, and phenylephrine in local pharmaceutical preparations by micellar electrokinetic chromatography
Author(s) -
Gallego José María Lemus,
Pérez Arroyo Julián
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301507
Subject(s) - chromatography , micellar electrokinetic chromatography , chemistry , repeatability , reproducibility , detection limit , calibration curve , chemometrics , dosage form , analytical chemistry (journal)
A new, rapid, and simple method is described and used to resolve and quantify mixtures of prednisolone, naphazoline, and phenylephrine. The determination was accomplished by micellar electrokinetic chromatography (MEKC) using a fused‐silica capillary (57 cm×75 μm ID). The separation was carried out at 25°C and 30 kV, using a 5 mM phosphate‐5 mM borate buffer adjusted to pH = 8.2, 40 mM sodium dodecylsulfate (SDS) as background electrolyte. Under these conditions, the run time was 6.6 min and the limits of quantification were about 0.4 mg/L for every component. Repeatability and reproducibility studies showed no significant differences at 95% confidence level. Application of multivariate calibration regression spectrophotometric methods (PLS‐1, PLS‐2, and PCR) clearly demonstrated, especially in the case of PLS‐1, the high resolving powder of these techniques if all possible interferences are suppressed. MEKC has been used for quantifying these compounds in different pharmaceutical products and the method gave good results compared with spectrophotometry. The pharmaceutical preparations do not require any separation steps when analysed by the two procedures described.