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Prediction of gas chromatographic retention times of capillary columns of different inside diameters
Author(s) -
Aryusuk Kornkanok,
Krisnangkura Kanit
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301505
Subject(s) - capillary action , chemistry , chromatography , analytical chemistry (journal) , logarithm , elution , thermodynamics , mathematics , physics , mathematical analysis
The retention times ( t R ) of n ‐alkanes (C 16 –C 22 ) eluted from capillary columns of different diameters are accurately predicted by using the equation proposed by Krisnangkura et al. ( J. Chromatogr. Sci. 1997 , 35 , 329–332). The numerical values of four thermodynamically related constants ( a , b , c , and d ) of the BP‐1 (100% dimethylpolysiloxane) capillary column (0.32 mm ID×25 m, film thickness, 0.25 μm) are –6.169, –0.512, 226.98, and 410.30, respectively. For columns of the same stationary phase but of different inside diameters and film thickness, accurate t R values can be predicted by using the same numerical values of the last three constants but the first constant ( a ) is changed by the difference in the natural logarithm of the column phase ratios (β). All the derived numerical values of each column were tested with FAMEs and with n ‐alkanes in temperature‐programmed GC (TPGC). All the predicted t R values agree well with the experimental values. About 77% of the TPGC data have errors lower than 0.5% and the largest value is –1.04%.