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Capillary electrophoresis profiles of fucoidan and heparin fractions: significance of mobility dispersity for their characterization
Author(s) -
Kim Moo Young,
Varenne Anne,
Daniel Régis,
Gareil Pierre
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301497
Subject(s) - absorbance , capillary electrophoresis , chemistry , electrophoresis , dispersity , chromatography , fucoidan , analytical chemistry (journal) , polysaccharide , ionic strength , organic chemistry , aqueous solution
Capillary electrophoresis was investigated for characterization of the natural anionic polysaccharide fucoidan from marine brown algae and its comparison with heparin under different pH, ionic strength, and counter‐ion conditions. Reproducible profiles of these polysaccharides were obtained in indirect UV detection mode by introducing sulfosalicylic acid or anisic acid as chromophores in background electrolytes. Theoretical evaluations of zone spreading originating from molecular diffusion, electromigration, and initial zone width have shown that these dispersive contributions remain negligible compared to the overall profile dispersion. Therefore, the conversion of the experimental electropherograms (absorbance vs. time plots) into absorbance vs. electrophoretic mobility curves enables a comparison of these polysaccharides in terms of electrophoretic mobility dispersity. Nevertheless, a linear correlation between mobility and chemical charge rate cannot be established for these highly sulfated polysaccharides, because their chemical charge rate is higher than the threshold value for which counter‐ion condensation occurs.