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Detection of proline, arginine, and lysine using iodine‐azide reaction in TLC and HPTLC
Author(s) -
Zakrzewski Robert,
Ciesielski Witold,
Kaźmierczak Dorota
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301452
Subject(s) - chemistry , chromatography , lysine , azide , iodine , arginine , proline , biochemistry , amino acid , organic chemistry
The application of a modified iodine‐azide procedure for the detection of proline, arginine, and lysine is described. Phenyl isothiocyanate was used to transform amino acids into phenyl thiocarbamyl derivatives (derivatization in situ). The developed plates were sprayed with a mixture of sodium azide and starch solution, adjusted to pH 5.5, and exposed to iodine vapour. Due to the catalytic effect of the CS bond, the spots appeared white on a violet‐grey background and were stable for 20 minutes. The detection limits were found to lie in the pmole range. The iodine‐azide test is compared with other procedures (iodine, UV, ninhydrin).