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Purification of peanut proteins for further use in affinity chromatography and as immunogens
Author(s) -
Baumgartner Sabine,
Hemetsberger Claudia,
Drs Elisabeth,
Pichler Harald,
Krska Rudolf
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301433
Subject(s) - affinity chromatography , chromatography , chemistry , ion chromatography , fraction (chemistry) , protein purification , tandem affinity purification , column chromatography , biochemistry , enzyme
Chickens were immunised with peanut protein extracts. Because of the high cross reactivity, the lgY antibodies were purified by means of an affinity column. An ion chromatographically purified peanut protein fraction was therefore coupled to the affinity support material and this column was used for further lgY purification.