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Analysis of 3‐quinuclidinol derivatives by capillary electrophoresis
Author(s) -
Bednář Petr,
Barták Petr,
Lemr Karel,
Ševčík Juraj,
Stránský Zdeněk
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301380
Subject(s) - chemistry , capillary electrophoresis , chromatography , analyte , electrolyte , derivative (finance) , imidazole , isopropyl , electrophoresis , potassium , polyethylene glycol , analytical chemistry (journal) , organic chemistry , electrode , financial economics , economics
Capillary electrophoresis with indirect UV detection was applied to the analysis of a mixture of 3‐quinuclidinol and its four quaternary derivatives ( N ‐methyl, N ‐ethyl, N ‐propyl, and N ‐isopropyl derivative). 10 mM imidazole acetate buffer, pH = 4.0 offers suitable detection sensitivity (LOD = 1 μmol L –1 ) and permits separation of the mixture except for the pair 3‐quinuclidinol– N ‐methyl derivative. The separation of all analytes was achieved on addition of 15 mmol L –1 β‐cyclodextrin or 25% ( w/w ) polyethylene glycol 2000 to the background electrolyte. The optimized method was employed for the analysis of pond water spiked with these analytes. Actual ionic mobilities of the studied compounds were measured using mobility standards (potassium, sodium, tetramethyl‐ and tetrabutylammonium). The migration index was derived as another identification parameter based on migration data and the precision of the obtained values is discussed in brief.