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Analysis of aristolochic acids in herbal medicines by LC/UV and LC/MS
Author(s) -
Lee MingChung,
Tsao ChiHsiang,
Iou SongChou,
Chuang WuChang,
Sheu ShuennJyi
Publication year - 2003
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200301271
Subject(s) - aristolochic acid , chromatography , chemistry , mass spectrometry , acetonitrile , electrospray ionization , solvent , electrospray , high performance liquid chromatography , organic chemistry , genetics , biology
Abstract Two high‐performance liquid chromatographic methods, one combined with a photodiode array detector (LC/UV) and another with mass spectrometry (LC/MS), were developed for the analysis of aristolochic acid I and II in herbal medicines. The LC/UV method was carried out using a Cosmosil 5C18‐MS column with a gradient solvent system composed of phosphate buffer‐acetonitrile and a UV detector (390 nm). The LC/MS method was performed using an acetate buffer‐acetonitrile solvent system and positive‐ion electrospray ionization MS. The characteristic fragment ions for aristolochic acid I were selected at m/z 359, m/z 324, m/z 298, and m/z 296, and for aristolochic acid II at m/z 329, m/z 294, and m/z 268. In order to attain better resolution, solid phase extraction was used to prepare the test solution in this study.