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Identification of very long chain fatty acids by atmospheric pressure chemical ionization liquid chromatography‐mass spectroscopy from green alga Chlorella kessleri
Author(s) -
Rězanka Tomáš
Publication year - 2002
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.200290004
Subject(s) - atmospheric pressure chemical ionization , chemistry , chromatography , mass spectrometry , chemical ionization , high performance liquid chromatography , chlorella , liquid chromatography–mass spectrometry , algae , ionization , organic chemistry , botany , biology , ion
A method is described for the enrichment of very long chain fatty acids (VLCFAs) from total fatty acids of heterotrophically cultivated green freshwater alga Chlorella kessleri and their identification as picolinyl esters by means of liquid chromatography‐mass spectrometry with atmospheric pressure chemical ionization (LC‐MS with APCI). The method is based on the use of preparative reversed phase HPLC of hundred‐milligram amounts and their subsequent identification by microbore APCI LC‐MS. A combination of these two techniques was used to identify unusual VLCFAs up to C 47 , both saturated and monounsaturated, with two positional isomers (ω‐9 and ω‐26).