Open AccessProtocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc tissues
Author(s) -
Veras Matthew A.,
Lim Yong J.,
Kuljanin Miljan,
Lajoie Gilles A.,
Urquhart Bradley L.,
Séguin Cheryle A.
Publication year - 2020
Publication title -
jor spine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
0ISSN - 2572-1143
DOI - 10.1002/jsp2.1099
Subject(s) - metabolomics , protocol (science) , proteomics , computational biology , tissue sample , modalities , omics , intervertebral disc , computer science , bioinformatics , quantitative proteomics , sample (material) , biology , pathology , medicine , chemistry , anatomy , biochemistry , social science , alternative medicine , sociology , gene , chromatography
The comprehensiveness of data collected by “omics” modalities has demonstrated the ability to drastically transform our understanding of the molecular mechanisms of chronic, complex diseases such as musculoskeletal pathologies, how biomarkers are identified, and how therapeutic targets are developed. Standardization of protocols will enable comparisons between findings reported by multiple research groups and move the application of these technologies forward. Herein, we describe a protocol for parallel proteomic and metabolomic analysis of mouse intervertebral disc (IVD) tissues, building from the combined expertise of our collaborative team. This protocol covers dissection of murine IVD tissues, sample isolation, and data analysis for both proteomics and metabolomics applications. The protocol presented below was optimized to maximize the utility of a mouse model for “omics” applications, accounting for the challenges associated with the small starting quantity of sample due to small tissue size as well as the extracellular matrix‐rich nature of the tissue.