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Absorption cytometric dna analysis of sections of gastric carcinomas and a comparison with cytofluorometry of single‐cell suspensions and flow cytometry
Author(s) -
Haraguchi Masaru,
Kakeji Yoshihiro,
Orita Hiroyuki,
Korenaga Daisuke,
Maehara Yoshihiko,
Sugimachi Keizo
Publication year - 1995
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930600312
Subject(s) - flow cytometry , ploidy , dna , aneuploidy , microbiology and biotechnology , cytometry , cell , pathology , biology , medicine , genetics , chromosome , gene
Abstract Measurements were made of cellular deoxyribonucleic acid (DNA) content in paraffin‐embedded materials from 44 gastric carcinomas, using three different methods; static cytometric light absorbance technique using tissue sections (SCM‐abs), static cytometric fluorescence technique using single‐cell suspensions (SCM‐flu), and flow cytometry (FCM). The DNA ploidy determined by SCM‐abs was grouped into low or high ploidy according to dispersion on the DNA histogram, and the DNA ploidy determined by SCM‐flu and FCM was grouped into diploidy or aneu‐ploidy, according to the peak DNA value. Accord between SCM‐abs and SCM‐flu or between SCM‐abs and FCM was confirmed when low ploidy by SCM‐abs showed diploidy by SCM‐flu or FCM and high ploidy by SCM‐abs showed aneuploidy by SCM‐flu or FCM. An incomplete but good accord of DNA ploidy was obtained with these methods. © 1995 Wiley‐Liss, Inc.