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Growth inhibition of intracerebral rat glioma by transfection‐induced human interferon‐β
Author(s) -
Harada Kunyu,
Yoshida Jun,
Mizuno Masaaki,
Kurisu Kaoru,
Uozumi Tohru
Publication year - 1995
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930590207
Subject(s) - liposome , transfection , glioma , interferon , medicine , cancer research , genetic enhancement , growth inhibition , pathology , gene , immunology , in vitro , biology , biochemistry
Abstract In our previous study on liposome‐mediated transfection of the human interferon‐β (HuIFN‐β) gene into subcutaneously implanted human gliomas in nude mice, we found that HuIFN‐β was produced and secreted by the tumor cells and that the growth of solid tumors was completely inhibited. The present study investigated the growth‐inhibitory effect of liposomes containing the HuIFN‐β gene inserted into a vector (PSV2IFN‐β) on T9 rat glioma implanted into the brains of rats. Tumor cells and liposomes containing pSV2IFN‐β or other additives were simultaneously injected into the brains of rats. HuIFN‐β was detected in solid gliomas growing in the brains of rats injected with liposomes and magnetic resonance imaging (MRI) showed that tumor growth was inhibited. In addition, the latent period until the appearance of neurological symptoms was significantly prologed in rats treated with liposomes containing pSV2IFN‐β. However, the survival time of the treated rats was not significantly increased. © 1995 Wiley‐Liss, Inc.