Premium
Studies of rhodamine‐123: Effect on rat prostate cancer and human prostate cancer cells in vitro
Author(s) -
Arcadi John A.,
Shankar Narayan K.,
Techy Geza,
Ng ChuenPei,
Saroufeem Ramez M. G.,
Jones Lawrence W.
Publication year - 1995
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930590204
Subject(s) - clonogenic assay , prostate cancer , medicine , prostate , rhodamine 123 , cancer , in vitro , cancer cell , cytotoxic t cell , cancer research , pathology , biology , biochemistry , multiple drug resistance , antibiotics
The effect of the lipophilic, cationic dye, Rhodamine‐123 (Rh‐123), on prostate cancer in rats, and on three tumor cell lines in vitro is reported here. The general toxicity of Rh‐123 in mice has been found to be minimal. Lobund‐Wistar (L‐W) rats with the autochthonous prostate cancer of Pollard were treated for six doses with Rh‐123 at a dose of 15 mg/kg subcutaneously every other day. Microscopic examination of the tumors revealed cellular and acinar destruction. The effectiveness of Rh‐123 as a cytotoxic agent was tested by clonogenic and viability assays in vitro with three human prostate cancer cell lines. Severe (60‐95%) growth inhibition was observed following Rh‐123 exposure for 2–5 days at doses as low as 1.6 μg/ml in all three prostate cancer cell lines.