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DNA ploidy and PCNA index in pancreatic lesions producing hyperinsulinemic hypoglycemia
Author(s) -
Losada J.,
Sarria R.,
Fernandez Val J. F.,
Lopez Ariztegui M. A.
Publication year - 1995
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930590105
Subject(s) - proliferating cell nuclear antigen , hyperinsulinemic hypoglycemia , pathology , medicine , lymph node , flow cytometry , lymph , pancreas , cytometry , hypoglycemia , immunohistochemistry , immunology , insulin
DNA nuclear content and PCNA index (proportion of PCNA reactive cells) have been studied by flow cytometry in eight pancreatic lesions producing hyperinsulinemic hypoglycemia to assess DNA ploidy and tumoral growth fraction. Adult nesidioblastosis had a diploid DNA index, two adenomas were near diploid, and four of the adenomas and the carcinoma were aneuploid. The median value of PCNA index (6.056% ± 6.76) was significantly correlated ( p <0.05) with the mean tumor diameter (2.43 cm ± 1.96). Tumors with a PCNA index <6.056% showed a diameter less than the mean tumoral diameter and a benign clinical course. Tumors with PCNA index <6.056% generally displayed a diameter bigger than the mean tumoral diameter, being associated with lymph node metastases in one case. The authors conclude that nuclear DNA and PCNA index cytometric studies are useful parameters to assess the biological behaviour of pancreatic lesions producing hyperinsulinemic hypoglycemia.