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Immunohistochemical analysis of p53 in colorectal cancer regarding clinicopathological correlation and prognostic significance
Author(s) -
Tomoda Hirotsugu,
Kakeji Yoshihiro
Publication year - 1995
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930580211
Subject(s) - medicine , immunohistochemistry , colorectal cancer , cancer , oncology , pathology , correlation , geometry , mathematics
Abstract The overexpression of the tumor suppressor gene p53 was investigated immunohistochemically in 144 cases of primary colorectal cancer and in 8 cases with cancer in the corresponding metastatic lymph nodes. Abnormalities in p53 expression were found in 36 cases (25%) of the 144 primary cancer cases. In addition, p53‐positive tumors were found to metastasize frequently to the lymph nodes, as compared to p53‐negative tumors (61.1% vs. 41.7%, P =0.0428). p53 staining was identical in 7 of 8 (87.5%) cases in primary and metastatic lesions. When the DNA content of the tumor was determined by flow cytometry, the DNA index (mean ± SD) was significantly higher in p53‐positive tumors than in p53‐negative tumors (1.57 ± 0.38 vs. 1.39 ± 0.37, P =0.012). Therefore, the immunohistochemical data of p53 in colorectal cancer may help in potentially predicting metastatic spread to the lymph nodes. © 1995 Wiley‐Liss, Inc.