Premium
Effects of preoperative chemotherapy on DNA ploidy patterns, cell cycle, and histological findings in gastric and colonic cancer patients
Author(s) -
Kubota Keiichi,
Konishi Toshiro,
Teruya Masanori,
Makuuchi Masatoshi,
Oka Teruaki,
Kajiura Naoaki,
Tsushima Hidefumi,
Nakao Kentaro,
Arizono Saori
Publication year - 1994
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930570404
Subject(s) - medicine , chemotherapy , cancer , colorectal cancer , biopsy , pathology , gastroenterology , cell cycle , tegafur , cell , biology , genetics
The effects of preoperative chemotherapy on gastric and colonic cancers have yet to be evaluated fully. In this study, its effects were assessed by studying DNA ploidy patterns, cell cycles, and histological findings in such patients. Thirty‐nine patients with gastric or colonic cancer were given preoperative chemotherapy with UFT (an admixture of tegafur and uracil). Biopsy specimens for analysis were obtained before chemotherapy through a gastroscope or colonoscope and after chemotherapy from resected tumors. The DNA ploidy patterns and cell cycles were evaluated using a flow cytometer and the tissues were examined histologically. The DNA ploidy pattern was diploid (D) in 12 gastric and 13 colonic cancer patients and aneuploid (A) in 10 and 4 patients, respectively. After chemotherapy, the pattern changed in nine gastric (A→D: 7, D→A: 2) and six colonic cancer patients (A→D: 3, D→A: 3) and was unchanged in the remaining patients. Cell cycle analysis showed decreased Gl‐ and increased S‐phase fractions in 10 of 12 patients with gastric and 6 of 10 patients with colonic cancer. Histologically, decreased tumor cellularity, increased fibrosis, and/or cytological changes were observed in both cancers after chemotherapy. Gastric and colonic cancers in which the DNA ploidy pattern changed from aneuploid to diploid, G1‐ decreased and S‐phase increased, and/or histological changes were observed, were considered to have responded to preoperative UFT administration. © 1994 Wiley‐Liss, Inc.