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Clostridium difficile toxin a therapy for hct 116 human colon cancer in nude mice
Author(s) -
Kushnaryov Vladimir M.,
Hernandez Irene,
Grossberg Sidney E.,
Redlich Philip N.
Publication year - 1994
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930570311
Subject(s) - medicine , clostridium difficile toxin a , toxin , in vivo , clostridium difficile , rash , colorectal cancer , immunotoxin , toxicity , cancer , pathology , pharmacology , gastroenterology , immunology , antibiotics , microbiology and biotechnology , biology , antibody , monoclonal antibody
Clostridium difficile toxin A was evaluated for an antitumor effect in vivo on HCT 116 human colon carcinoma cells growing subcutaneously in nude mice. A mean reduction in tumor volume of at least 65%, by measurement in three dimensions, was observed in mice who received two 9‐ to 13‐day courses of daily intraperitoneal injections of toxin A as compared to mice receiving diluent alone. Reversible adverse effects of toxin A were noted in some animals, consisting primarily of liver toxicity and skin rash. HCT 116 cells in toxin A‐treated mice grew as flattened tumors with ulcerated centers compared to rounded tumors without ulceration in controls. Histologic examination of tumors from representative mice revealed that two thirds of the tumor in a treated mouse was necrotic compared to only one third in a control, suggesting greater antitumor efficacy of toxin A than estimated by tumor measurements alone. © 1994 Wiley‐Liss, Inc.