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Survival and toxicity of xenogeneic murine retroviral vector producer cells in liver
Author(s) -
Moorman Donald W.,
Butler Daniel A.,
Stanley J. Daniel,
Lamsam Jesse L.,
Culver Kenneth W.,
Ackermann Mark R.,
Jacobson Carol D.
Publication year - 1994
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930570304
Subject(s) - medicine , toxicity , vector (molecular biology) , viral vector , virology , cancer research , gene , biology , recombinant dna , genetics
Murine retroviral vector producer cells (VPC) can selectively transfer genes stably into proliferating cells. We injected LacZ gene producing VPC directly into normal rat liver. There was no measurable gene transfer into the surrounding hepatic parenchyma with X‐GAL staining. Rejection of the xenogeneic murine VPC occurred 7‐14 days after injection. Toxicity of this delivery method was evaluated with the herpes simplex‐thymidine kinase (HS‐tk) gene, which confers sensitivity to the antiherpes drug, ganciclovir (GCV). HS‐tk VPC were injected and allowed to grow in normal liver for 7 days before GCV treatment. There was no clinical or histologic evidence of toxicity with GCV treatment. These findings suggest that the direct injection of murine VPC into xenogeneic human tumors may survive sufficiently long without immunosuppression to transfer genes to tumor cells in situ without attendant toxicity. © 1994 Wiley‐Liss, Inc.