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Alteration of tumorigenicity in undifferentiated thyroid carcinoma cells by introduction of normal chromosome 11
Author(s) -
Yoshida Akira,
Asaga Tarou,
Masuzawa Chihiro,
Kawahara Satoru
Publication year - 1994
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930550308
Subject(s) - chromosome , microbiology and biotechnology , chromosome 22 , chromosome 7 (human) , marker chromosome , cell culture , biology , cancer research , cell , thyroid carcinoma , chromosome abnormality , gene , thyroid , karyotype , genetics
The tumorigenicity of various cell lines has been shown to be suppressed by the introduction of chromosome 11 and other chromosomes via micro‐cell‐mediated chromosome transfer. In this study, we investigated whether a human undifferentiated thyroid carcinoma cell line, TTA‐1, was suppressed by the introduction of normal human chromosome 11 or 10. Chromosome 10 or 11 was transferred from A 9 cells containing a single human chromosome 10 or 11 tagged with pSV 2 ‐neo plasmid DNA into TTA‐1 cells, by microcell fusion. The tumorigenicity of the TTA‐1 cells and their colony‐forming efficiency in soft agar were suppressed by chromosome 11, but not by chromosome 10. These results suggest that normal human chromosome 11 carries a putative tumor suppressor gene that affects the tumor‐associated phenotypes of TTA‐1 cells. © Wiley‐Liss, Inc.