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Expression of proliferating cell nuclear antigen in Wilms' tumors and other pediatric renal tumors: The correlation between histologic classification and proliferative activity
Author(s) -
Nagoshi Makoto,
Tsuneyoshi Masazumi
Publication year - 1994
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930550211
Subject(s) - anaplasia , proliferating cell nuclear antigen , wilms' tumor , pathology , immunohistochemistry , medicine , nephrectomy , flow cytometry , kidney , immunology
Abstract In order to estimate the proliferative activities in each prognosis predicting histologic type immunohistochemical studies for proliferating cell nuclear antigen (PCNA) have been performed on 27 pediatric renal tumors using formalin‐fixed paraffin‐embedded specimens. Image analyses determined the percentage of positive areas for PCNA, which were then represented in the PCNA score. The PCNA scores of nephroblastomas without anaplasia roughly correlated with the S phase fractions obtained by flow cytometry. The PCNA scores for each tumor were as follows; the renal specimens for the control study (n = 10, a mean ±SSD of 8.04 ± 3.59%); the nephroblastomas without anaplasia (n = 15, 27.70 ± 7.59%); the nephroblastomas with anaplasia (n = 3, 36.89 ± 1.17%); the sarcomatous renal tumors (n = 5, 27.75 ± 5.02); the renal cell carcinomas (n = 2, 28.31 ± 0.86%), a congenital mesoblastic nephroma and a juxtaglomerular cell tumor (10.05 and 10.80%, respectively). The PCNA scores of the pediatric renal tumors correlated with neither the outcome of the patients nor the histologic groups in predicting the prognosis for such pediatric renal tumors. © Wiley‐Liss, Inc.