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Proliferative activity of gastric cancer assessed by immunostaining for proliferating
Author(s) -
Inada Takao,
Imura Joji,
Ichikawa Akira,
Ogata Yoshiro,
Shimamura Kayako
Publication year - 1993
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930540303
Subject(s) - proliferating cell nuclear antigen , immunostaining , pathology , flow cytometry , carcinoembryonic antigen , staining , adenocarcinoma , metastasis , immunohistochemistry , lymph node , antigen , monoclonal antibody , medicine , cancer , microbiology and biotechnology , biology , antibody , immunology
The growth activity of 107 gastric carcinomas was assessed by immunohis‐tochemical staining for formalin‐fixed, paraffin‐embedded tissue with a monoclonal antibody against proliferating cell nuclear antigen (PCNA). When the tumor doubling times (Tds) of 10 patients were estimated from the serum levels of carcinoembryonic antigen and carbohydrate antigen 19–9, there was an inverse correlation between the Tds and PCNA labeling index (LI) at P = 0.055. Flow‐cytometric analysis was carried out by double staining for PCNA and DNA using fresh materials from 14 patients. The PCNA‐positive cell fraction revealed by flow cytometry showed a good linear correlation with PCNA LI in routinely stained tissue. The LI of well‐differentiated adenocarcinoma was significantly higher than that of the poorly differentiated type. When the LI was analyzed in well‐ or poorly differentiated adenocarcinoma, the value was significantly higher in the well‐differentiated type with hepatic metastasis and in the poorly differentiated type with lymph node metastasis. © 1993 Wiley‐Liss, Inc.