Premium
Photo detection of carcinoma of the colon in a rat model: A pilot study
Author(s) -
von Rueden David G.,
McBrearty Francis X.,
Clements Barton M.,
Woratyla Steven
Publication year - 1993
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930530112
Subject(s) - medicine , colon carcinoma , carcinoma , colorectal cancer , oncology , general surgery , surgery , cancer
Photodynamic sensitizers are light‐absorbing chemicals that cause photo‐reactions in biologic systems when exposed to light of the proper wavelength. Dihematoporphyrin ethers (DHE) are the active porphyrin derivatives most commonly used as a photosensitizer (Photofrin, QLT). DHE accumulates in tumor tissue and also fluoresces when light activated. A more reliable and less costly screening method for early detection and treatment of colon cancer is needed. The present study was designed to induce adenocarcinoma of the colon in rats with 1,2 dimethylhydrazine (DMH) and attempt to identify tumors early in their evolution by DHE fluorescence. Forty rats were injected with 20 mg/kg of DMH at weekly intervals until sacrifice. Photofrin (3 mg/kg) was injected through the tail vein in each prior to sacrifice. Eight colonic specimens contained invasive adenocarcinoma, seven of which fluoresced when exposed to a Woods lamp. Carcinoma in situ was identified in two specimens by fluorescence, and one fluorescent specimen contained dysplasia. © 1993 Wiley‐Liss, Inc.