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Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases
Author(s) -
Furukawa Toshiharu,
Watanabe Masahiko,
Kubota Tetsuro,
Kase Suguru,
Fujita Shin,
Yamamoto Takaaki,
Saikawa Yoshiro,
Kuo TsongHong,
Tanino Hirokazu,
Kurihara Naoto,
Kawano Yukio,
Kawamoto Kiyoshi,
Suto Akihiko,
Teramoto Tatsuo,
Ishibiki Kyuya,
Kitajima Masaki
Publication year - 1993
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930530105
Subject(s) - medicine , extracellular matrix , in vitro , clinical significance , colorectal cancer , extracellular , pathology , cancer research , metastasis , cancer , microbiology and biotechnology , biochemistry , biology
The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)—Matrigel, laminin, fibronectin, and type IV collagen—was evaluated using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers. © 1993 Wiley‐Liss, Inc.