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Heterogeneity in early and advanced gastric carcinomas by flow cytometric DNA analysis
Author(s) -
Umehara Yasuhiko,
Kimura Taizou,
Yoshida Masayuki,
Oba Noriyuki,
Harada Yukio
Publication year - 1993
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930520208
Subject(s) - ploidy , cancer , flow cytometry , dna , pathology , aneuploidy , medicine , biology , oncology , microbiology and biotechnology , genetics , chromosome , gene
We investigated 230 systematically sampled fresh specimens from 12 early and 26 advanced gastric cancer patients by DNA flow cytometry for heterogeneity in DNA content. Fifty‐eight percent of the 12 early gastric cancers were uniformly diploid and 42% were uniformly aneuploid. Fifty‐four percent of advanced cancers were uniformly diploid in superficial layers and 42% were uniformly diploid in deep layers, whereas 46% were uniformly aneuploid in superficial layers, and 50% were uniformly aneuploid and 8% were heterogeneously aneuploid and diploid in deep layers. Both diploid and aneuploid samples were obtained from 15% for advanced cancers, but ploidy heterogeneity did not occur in early cancers. Heterogeneity for DNA index (more than one aneuploid DNA index) occurred in 46% of whole thickness of advanced cancers, in 19% of superficial layers of advanced cancers, and in 8% of early cancers. We concluded that DNA ploidy determination using superficial layer specimens may be reliable in early gastric cancer but must be interpreted with care in advanced cancer. © 1993 Wiley‐Liss, Inc.