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Colorimetric chemosensitivity testing using sulforhodamine B
Author(s) -
Kubota Tetsuro,
Takahara Tetsuya,
Nagata Midori,
Furukawa Toshiharu,
Kase Suguru,
Tanino Hirokazu,
Ishibiki Kyuya,
Kitajima Masaki
Publication year - 1993
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930520205
Subject(s) - sulforhodamine b , chemosensitivity assay , mitomycin c , cell culture , doxorubicin , microbiology and biotechnology , gentamicin protection assay , clonogenic assay , in vivo , medicine , cell counting , reproducibility , cisplatin , bradford protein assay , cytotoxicity , cell , in vitro , chromatography , tumor cells , cancer research , biology , chemotherapy , chemistry , biochemistry , cancer , surgery , cell cycle , genetics , metastasis
A colorimetric chemosensitivity test was investigated using sulforhodamine B (SRB), which stains protein synthesized by cells, as an end‐point marker. Four cultured cell lines, 9 human tumor xenografts serially transplanted into nude mice, and 14 fresh surgical specimens were subjected to this assay. The optimal conditions for the assay were 3–5 × 10 4 cells per well in a 96‐microplate, an SRB concentration of 4%, and an incubation time of more than 10 minutes. When mitomycin C, doxorubicin, cisplatin, and 5‐fluorouracil were assessed by the SRB assay, the concentration‐effect curves revealed a sharp slope between plateaux at low and high concentrations, suggesting that this assay has an excellent sensitivity which can assess the effect of drugs as “all or none.” Although this high sensitivity resulted in good reproducibility of the assay for cultured cell lines, the predictive rate of the SRB assay for the chemosensitivity of human tumor xenografts in vivo was limited to 63.9%. As a result, this SRB assay is thought to be useful for evaluating the chemosensitivity of cultured cells as all or none, since it can assess directly cellular protein synthesis, which is one of the most important parameters of cell renewal, with excellent sensitivity. © 1993 Wiley‐Liss, Inc.