Premium
Proliferative activity in gastric cancer determined with cell cycle‐related monoclonal antibodies Ki‐67 and p105: Analysis by flow cytometry
Author(s) -
Kimura Hironobu,
Yonemura Yutaka,
Miyazaki Itsuo
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930510310
Subject(s) - flow cytometry , monoclonal antibody , medicine , ki 67 , antigen , metastasis , cancer , microbiology and biotechnology , antibody , pathology , cell cycle , cancer research , immunology , biology , immunohistochemistry
The proliferative activity of gastric cancer cells was determined by DNA flow cytometric (FCM) analysis and labeling rates of Ki‐67 and monoclonal antibodies and proliferation‐associated nuclear antigen (p105) au‐toantibodies in 28 patients with fresh human gastric cancer cells. By setting the cutoff line at the level as used in a negative control study without primary antibody in the same sample, the Ki‐67 and p105 labeling rates were calculated by the dual fluorescence analysis. A total of 43 experiments was performed on FCM analysis for each antigen: 28 with Ki‐67 and 15 with p105. The mean Ki‐67 labeling rate of gastric cancer cells was 45.1% (13.9‐76.3%). The Ki‐67 labeling rates were significantly higher for larger size tumor, peritoneal metastasis, and advanced clinical stage. A significant correlation was found between Ki‐67 labeling rate and p105 labeling rate ( P < 0.05). Bivariate FCM may be an easy method for obtaining useful information of cell kinetics. © 1992 Wiley‐Liss, Inc.