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Prognostic assessment of superficial squamous cell carcinoma of the esophagus using karyometric analysis and nucleolar organizer regions
Author(s) -
Maesawa Chihaya,
Masuda Tomoyuki,
Tamura Gen,
Satodate Ryolchl,
Ishida Kaoru,
Saito Kazuyoshi
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930510308
Subject(s) - medicine , esophagus , basal cell , pathology , carcinoma , oncology , anatomy
To investigate prognostic values for squamous cell carcinoma, we measured nuclear area (NA), nuclear shape factor (NSF), DNA content and mean number of nucleolar organizer regions (NOR), using formalin‐fixed, paraffin‐embedded tissue sections from 39 patients with superficial squamous cell carcinoma of the esophagus. NA and DNA content were significantly higher in patients with lymph node metastasis than in those without metastasis. NA and the NOR number were also significantly higher in patients with recurrence than in those surviving for more than 3 years without recurrence. To determine the optimal combination of these parameters for prognostic assessment, we used a stepwise discriminant analysis to obtain two linear discriminant functions. One (f = −0.331 × NA + 6.64 × NSF + 9.85) gave an accuracy of 87.2% in predicting lymph node metastasis, and the other (f = −0.071 × NA −2.34 × NOR + 13.0) yielded an overall accuracy of 88.9% in classifying patients into two groups with a better and worse prognosis. These results suggest that karyometric analysis and determination of the NOR number are useful for the prediction of disease outcome in patients with superficial squamous cell carcinoma of the esophagus. © 1992 Wiley‐Liss, Inc.