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Effect of liposomal interleukin‐2 on ascites‐forming rat hepatoma
Author(s) -
Konno Hiroyuki,
Maruo Yuji,
Matin A. F.,
Tanaka Tatsuo,
Nakamura Satoshi,
Baba Shozo,
Yamashita Akira,
Tadakuma Takushi
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930510110
Subject(s) - ascites , medicine , liposome , tumor necrosis factor alpha , saline , radical , interleukin , intraperitoneal injection , endocrinology , pharmacology , cytokine , biochemistry , chemistry
Interleukin‐2 was entrapped in liposomes (Lip‐IL‐2) and injected into rats. The intraperitoneal injection of Lip‐IL‐2 into rats bearing an ascites‐forming rat hepatoma (AH‐66) significantly increased the survival time when compared with rats administered free IL‐2 or saline‐containing liposomes. The number of peritoneal excudate cells (PEC) increased markedly after intraperitoneal injection of Lip‐IL‐2 and consisted mainly of macrophages. The level of tumor necrosis factor a (TNF‐α) and the intensity of free radicals increased in the ascites at 48 hrs after Lip‐IL‐2 administration, whereas TNF‐α was not detected and the intensity of free radicals did not increase after free IL‐2 administration. Our findings suggested that entrapment of IL‐2 into liposomes enhanced its potential for cancer therapy, presumably by activating macrophages to produce TNF‐α and free radicals. © 1992 Wiley‐Liss, Inc.