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Investigation of colon cancers for human papillomavirus genomic sequences by polymerase chain reaction
Author(s) -
Shah Keerti V.,
Daniel Richard W.,
Simons Jonathan W.,
Vogelstein Bert
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930510104
Subject(s) - polymerase chain reaction , human papillomavirus , papillomaviridae , medicine , virology , colorectal cancer , cancer research , biology , gene , genetics , cancer , cervical cancer
Abstract Two previous studies have reported the presence of human papillomavirus (HPV) genomic sequences in colorectal cancers. We examined DNAs from 50 colorectal tumors for HPV sequences by polymerase chain reaction (PCR), using HPV consensus L1 primers for amplification. The PCR products were screened with a generic HPV probe consisting of amplimers of HPV‐16 and HPV‐18. All tumor DNAs were negative for HPV sequences. These results call into the question the reported association of colorectal cancers with HPVs. © 1992 Wiley‐Liss, Inc.