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Accuracy of the bronchoscopic DNA content analysis of non‐small‐cell lung carcinoma
Author(s) -
Haneda Hitoshi,
Miyamoto Hiroshi,
Isobe Hiroshi,
Abe Shosaku,
Akita Hirotoshi,
Ishikuro Akihiko,
Kawakami Yoshikazu,
Okayasu Kenji,
Endo Takashi,
Inoue Kazuaki
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930490311
Subject(s) - aneuploidy , concordance , dna , medicine , pathology , carcinoma , flow cytometry , microbiology and biotechnology , biology , immunology , chromosome , genetics , gene
To assess the accuracy of the bronchoscopic DNA content analysis, samples of non‐small‐cell lung carcinomas (NSCLC) were investigated by means of flow cytometry. Samples were dissociated using the detergent Triton X‐100. In 58 NSCLC cases, 39 (67%) had DNA aneuploid tumors. We compared the DNA indices of bronchoscopic brushing samples with 21 corresponding surgical samples. In 16 (76%) cases, DNA ploidy of both bronchoscopic and surgical samples were in concordance. In 3 (14%) cases, both bronchoscopic and surgical sample showed DNA aneuploidy, but the number of the DNA aneuploid stem cell lines was different. The cause of these differences was ascribed to the intratumor DNA heterogeneity. In 2 (10%) cases, the bronchoscopic sample showed DNA diploidy, but the surgical sample showed DNA aneuploidy. In these cases, tumor cells obtained by bronchoscopic brushing were so few that the small DNA aneuploid peak was undetectable in the DNA histogram. But the tumor DNA ploidy was evaluated correctly in 90% of 21 cases using bronchoscopic samples. Consequently, despite some drawbacks, the DNA ploidy diagnosis using bronchoscopic samples in this relatively small study, was almost as reliable as surgical samples.