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Distribution of 111 in‐bleomycin complex in small cell lung cancer cells by autoradiography
Author(s) -
Hou DeYan,
Maruyama Yosh
Publication year - 1992
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930490206
Subject(s) - bleomycin , cytoplasm , cell , distribution (mathematics) , nucleus , medicine , pathology , lung , cell membrane , microbiology and biotechnology , biology , biochemistry , chemotherapy , mathematics , psychiatry , mathematical analysis
The distribution of 111 In‐bleomycin Complex ( 111 In‐BLMC) in small cell lung cancer (SCLC) cells was studied by autoradiography. SCLC cells were exposed to 111 In‐BLMC and 111 Indium chloride ( 111 InCl 3 ) for 1 hour, 3 hours, and 4 hours; washed with fresh medium; and spread on slides. The slides were smeared with NTB 2 . (NTB 3 ) emulsion by wet or dry‐mount technique and exposed 3 to 15 days. 111 In‐BLMC was found to localize in the cell nucleus and nuclear membrane (78.3%); 111 InCl 3 located mainly in the cytoplasm (52.3%). This distribution of labeled BLM may explain the mechanism of killing SCLC cells by 111 In‐BLMC.