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In vitro response of a human colon tumor xenograft and a lung adenocarcinoma cell line to α‐difluoromethylornithine alone and in combination with 5‐fluorouracil and doxorubicin
Author(s) -
Zirvi Karimullah A.,
Atabek Umur
Publication year - 1991
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930480107
Subject(s) - medicine , adenocarcinoma , doxorubicin , cell culture , growth inhibition , a549 cell , cancer research , in vitro , pharmacology , lung , fluorouracil , chemotherapy , immunology , biology , cancer , biochemistry , genetics
We have investigated effects of α‐difluoromethylornithine (DFMO), both as a single agent and in combination with 5‐fluorouracil (5‐FU) against a human colon tumor xenograft (T6) grown as primary tissue culture in serum‐free medium and in combination with doxorubicin (DX) against a human lung adenocarcinoma cell line (A549). DFMO showed a dose‐dependent growth‐inhibitory effect against human colon tumor xenograft and lung adenocarcinoma cells. Growth‐inhibitory activity of DFMO against T6 cells was reversed completely when the cells were treated simultaneously with putrescine (PU) (10 −6 M) and DFMO (10 −3 M). When 5‐FU and DFMO were used in combination against T6 cells, no antagonism or synergism between the two drugs was seen. However, in the case of A549 cells, when DFMO was used in combination with DX, there was a consistent increase in growth inhibition that surpassed the inhibition of either agent given alone.