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Immune competent cells of regional lymph nodes in colorectal cancer patients: I. Flow cytometric analysis of lymphocyte subpopulation
Author(s) -
Adachi Wataru,
Sugenoya Akira,
Iida Futoshi
Publication year - 1991
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930460209
Subject(s) - colorectal cancer , lymph , medicine , immune system , flow cytometry , lymphocyte , cancer , cancer cell , immunology , antibody , t lymphocyte , pathology , cancer research
Lymphocyte subpopulations of the regional lymph nodes in 20 colorectal cancer patients were measured by flow cytometry to analyze nodal lymphocytes phenotypically. Eleven patients with cholelithiasis were used as controls. Comparison of lymphocyte subpopulations between colorectal cancer and control groups revealed a significantly increased rate of Leu‐12 + cells in the cancer patients. Furthermore, a significant increase of Leu‐3a + Leu‐8 − cells and a significant decrease of Leu‐3a + Leu‐8 + cells were also found in the paracolic nodes of the cancer patients. However, Leu‐2a + Leu‐15 − cells were not different between the both groups. Meanwhile, in the cancer patients, the rates of Leu‐4 + , Leu‐3a + , and Leu‐3a + Leu‐8 − cells were higher in the paracolic nodes compared with the intermediate nodes. Additional investigation of the correlation between clinicopathological features of the tumors and lymphocyte subpopulations, showed that Leu‐12 + cells were related to the tumor size and Leu‐2a + 15 − cells related to the histopathological type. The foregoing results indicate that the rates of both B and helper T cells, which may specifically participate in the antibody production, increase in the regional lymph nodes of colorectal cancer patients.