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Gamma‐detecting probe and autoradiographic studies of radiolabeled antibody B72.3 in CX‐1 colon xenografts
Author(s) -
Sampsel James W.,
Hinkle George,
Nieroda Carol,
Ignaszewski Judi,
Thurston Marlin,
Martin Edward W.
Publication year - 1990
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.2930450407
Subject(s) - immunoperoxidase , monoclonal antibody , pathology , medicine , antibody , immunohistochemistry , nuclear medicine , immunology
Nude mice bearing CX‐1 colon tumors were injected with 50 μCi 125 I‐labeled monoclonal antibody (MAb) B72.3. Radioactivity in tumors was studied with the gamma detecting probe (GDP) on days 1, 3, 7, and 10 after MAb injection. On each day, two mice were sacrificed and sections were examined with autoradiography (ARG), immunoperoxidase methods (IMP), and routine stains. Mean probe counts showed increasing tumor to background ratios and ARG demonstrated a rogressive increase in radionuclide in the tumors. The distribution of 125 I was primarily around the vascular spaces on day 1, but by day 3 and progressively it appeared in tumor gland lumina and necrotic areas. A regional correlation was shown between radionuclide in vascular spaces and its sequestration in tumor elements.